The significance of blue polychromasia.

Materials and Methods As the polychromasic cells (Key, 1921; Rodan, 1949) and nucleated red cells (Stein, 1948; Rodan, 1949) collect in the buffy layer, a blind study was carried out on the buffy layer from 95 patients, including 11 children under the age of 6 years. A total of 137 slides (no more than two from each patient) was examined. With one exception (an iron deficiency anaemia) all slides examined represented the morphology of the red cells before treatment. The buffy layer was lifted with a Pasteur pipette from the standard haematocrit tubes. The contents of the pipette was divided between two slides and the